Calbiotech ANA Screen IgG Elisa Kit

Intended Use

The Calbiotech Inc. (CBI) ANA Screen Elisa test system is an enzyme-linked immunosorbent assay (Elisa ) for the detection of IgG class antibodies to ANA in human serum or plasma.

Principle Of The Test

Diluted patient serum is added to wells coated with purified nuclear antigens. ANA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.