DRG Corticosterone Elisa Kit

The DRG Corticosterone ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards a unique antigenic site of the corticosterone molecule. During the first incubation, the corticosterone in the added sample competes for the binding to the coated antibody with the added conjugate molecule, which is a Corticosterone molecule conjugated to a horse-radish peroxidase. After incubation the unbound conjugate and sample are washed off. Following the addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of corticosterone in the sample. After incubation of substrate solution, the colorimetric reaction is abruptly stopped by adding stop solution.