Calbiotech Testosterone Elisa Kit

INTENDED USE

The Calbiotech Inc. (CBI) ANA Screen ELISA test system is an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG class antibodies to ANA in human serum or plasma.

PRINCIPLE OF THE TEST

The Testosterone EIA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of mouse anti-Testosterone. l of TestosteronemIn the incubation, mouse anti-Testosterone coated wells are incubated with 25 l Testosterone-HRP conjugate reagent at roommstandards, controls, patient samples, 100  temperature for 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.