Elabscience Human CPP[Copeptin] Elisa Kit

INTENDED USE

This Elisa kit applies to the in vitro quantitative determination of Human CPP concentrations in serum, plasma and other biological fluids.

TEST PRINCIPLE

This Elisa kit uses the Competitive-Elisa  principle. The micro Elisa plate provided in this kit has been pre-coated with Human CPP. During the reaction, Human CPP in samples or Standard competes with a fixed amount of Human CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CPP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human CPP in the samples is then determined by comparing the OD of the samples to the standard curve.