Calbiotech T4 [Thyroxine] Elisa Kit

INTENDED USE

The Calbiotech Thyroxine (T4) ELISA Kit is intended for the detection of Total T4 in human serum or plasma.

Summary and explanation

T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The level of T4 is normal in Euthyroid individuals.

PRINCIPLE OF THE TEST

The CBI T4 is a solid phases competitive ELISA. The samples, working T4-HRP Conjugate and Anti-T4- Biotin Solution are added to the wells coated with Streptavidin. T4 in the patient’s serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.